Nucleic acid isolation - MRC
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| RNA ISOLATION | Cat.# | Quantity |
| RNAzol® RT Reagent A single-step reagent isolating mRNA and small RNA in separate fractions. No chloroform-induced phase separation or refrigerated centrifuge required. No DNase treatment. Higher yields and quality than previous single-step methods. |
RN 190 | 50 ml 100 ml 200 ml 500 ml |
| RNAzol® BD Reagent A single-step reagent isolating total RNA, or mRNA and small RNA in separate fractions from whole blood, plasma or serum. 1.0 ml of reagent processes 0.5 ml of blood. Higher yields and quality than previous methods. Simultaneous isolation of DNA. |
RB 192 | 50 ml 100 ml 200 ml 500 ml |
| DNA Extraction Solution After using RNAzol® BD to isolate RNA from blood samples, use DNA Extraction Solution to isolate genomic DNA from the organic phase. Simultaneous isolation of RNA and DNA from the same sample using RNAzol® BD and DNA Extraction Solution. |
GT 192 | 50 ml |
| TRI Reagent® For single-step isolation of RNA or simultaneous isolation of RNA, DNA and proteins from tissues or cells. 1 ml of TRI Reagent® processes up to 100 mg tissue or 107 cells. |
TR 118 | 50 ml 100 ml 200 ml 500 ml |
| TRI Reagent® BD For single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from whole blood, plasma, or serum. 0.75 ml of TRI Reagent® BD processes 0.25 ml of blood derivatives. |
TB 126 | 50 ml 100 ml 200 ml 500 ml |
| TRI Reagent® LS For single-step isolation of total RNA or simultaneous isolation of RNA, DNA and proteins from liquid samples. 0.75 ml of TRI Reagent® LS processes 0.25 ml of liquid samples. |
TS 120 | 50 ml 100 ml 200 ml 500 ml |
| TRI Reagent® RT A reagent for the single-step isolation of RNA from tissues and cells. 1 ml of TRI Reagent® RT processes 50 mg tissue or 107 cells. The isolated RNA is free of DNA contamination and ready for RT-PCR without the DNase treatment. |
RT 111 | 50 ml 100 ml 200 ml 500 ml |
| TRI Reagent® RT-Liquid Samples A reagent for the single-step isolation of RNA from liquid samples. 0.75 ml of TRI Reagent® RT processes 0.25 ml of liquid samples. The isolated RNA is free of DNA contamination and ready for RT-PCR without the DNase treatment. |
RL 311 | 50 ml 100 ml 200 ml 500 ml |
| BCP BCP is molecular biology grade 1-bromo-3-chloropropane for phase separation in the TRI Reagent® procedures. |
BP 151 | 200 ml |
| BAN BAN is molecular biology grade 4-bromoanisole for phase separation in the TRI Reagent® RT and RNAzol RT® procedures. |
BN 191 | 100 ml |
| Precipitation Carrier Molecular biology grade carrier for use in precipitation of mRNA fraction with RNAzol® RT. |
PC 173 | 5 ml |
| Polyacryl Carrier Molecular biology grade acryl polymer for use in precipitation of RNA and DNA. |
PC 152 | 5 ml |
| FORMAzol® (Stabilized formamide) RNA solubilizer that allows storage of RNA at -20°C, protects against RNase degradation and simplifies northern analysis. |
FO 121 | 25 ml |
| High Salt Precipitation Solution Eliminates polysaccharide and proteoglycan contamination from RNA in the TRI Reagent® procedure. |
PS 161 | 100 ml |
| DNA ISOLATION | Cat.# | Quantity |
| DNAzol® Genomic DNA isolation reagent for use with tissues, cells and liquids. The isolation is fast (10-30 min), simple and reliable. Non-toxic. 1 ml of DNAzol® isolates DNA from 25-50 mg tissue or 1-3 x 107 cells. |
DN 127 | 50 ml 100 ml 200 ml 500 ml |
| DNAzol® ES Extra Strength DNAzol® reagent for isolation of genomic DNA from plants. Non-toxic. 1.5 ml DNAzol® ES isolates DNA from 0.5 g of plant sample. |
DN 128 | 50 ml 100 ml 200 ml 500 ml |
| DNAzol® BD A DNAzol® reagent specifically designed for isolation of genomic DNA from whole blood. Non-toxic. 1 ml of DNAzol® BD isolates DNA from 0.5 ml of whole blood. |
DN 129 | 50 ml 100 ml 200 ml 500 ml |
| DNAzol® Direct A universal reagent for sample processing for direct PCR. Lyse sample in DNAzol® Direct, incubate and use lysate as PCR template. No DNA isolation necessary. Non-toxic. Use 50 µl reagent per 5 µl whole blood or 1 mg tissue. |
DN 131 | 25 ml 50 ml |
| Bactozol™ Kit A kit for the isolation of bacterial DNA. Kit includes 10X Bactozol™ Enzyme Solution, Dilution Buffer and DNAzol® sufficient for 125 isolations, each yielding up to 40 µg bacterial DNA. |
BA 154 | 125 isolations |
| Bactozol™ Enzyme Solution A solution stable at room temperature containing activated lysozyme for the effective lysis of a broad range of bacterial specimens. Sufficient for 125 lysis reactions, each with up to 40 µg bacterial DNA. |
BZ 160 | 125 reactions |
| Polyacryl Carrier Molecular biology grade acryl polymer for use in precipitation of RNA and DNA. |
PC 152 | 5 ml |